PENGARUH PEMBERIAN EKSTRAK BUAH DELIMA MERAH (Punica granatum) TERHADAP KADAR F2-IsoPs PADA KULTUR HUVECs YANG DIPAPAR PLASMA PREEKLAMPSIA

Januarsih; Megawati; Fitria Jannatul Laili

doi:10.62354/jurnalmedicare.v4i4.274

Penulis

Januarsih Poltekkes Kemenkes Banjarmasin
Megawati Poltekkes Kemenkes Banjarmasin
Fitria Jannatul Laili Poltekkes Kemenkes Banjarmasin

DOI:

https://doi.org/10.62354/jurnalmedicare.v4i4.274

Kata Kunci:

preeklampsia, F2-IsoPs, punica granatum

Abstrak

Preeclampsia is a leading cause of maternal mortality associated with oxidative stress. Elevated F2-Isoprostanes (F2-IsoPs) serve as biomarkers of lipid peroxidation. This study aimed to determine the effect of red pomegranate (Punica granatum) extract on F2-IsoPs levels in HUVEC cultures exposed to preeclamptic plasma. A true experimental post-test only control group design was used with five groups (n=5 each): K– (normal plasma), K+ (preeclamptic plasma), P1 (14 ppm), P2 (28 ppm), and P3 (56 ppm). F2-IsoPs levels were measured using ELISA and analyzed with one-way ANOVA and LSD post hoc tests. Results showed significantly higher F2-IsoPs levels in K+ compared to K– (p<0.05). Red pomegranate extract reduced F2-IsoPs levels in a dose-dependent manner, with the greatest reduction at 56 ppm. Conclusion: Punica granatum extract exhibits antioxidant potential against oxidative stress in preeclampsia.

Unduhan

Data unduhan belum tersedia.

Referensi

Asgary, S., Sahebkar, A., Afshani, M. R., Keshvari, M., Taheri, M., & Jahanian, E. (2024). Effects of pomegranate on vascular endothelial function: A systematic review and meta-analysis of clinical trials. Phytotherapy Research, 38(5), 2231–2243. https://doi.org/10.1002/ptr.8012

Januarsih, I., Dewi, R., & Sari, M. (2023). Punica granatum polyphenols modulate antioxidant enzymes in endothelial dysfunction models of Preeklampsi. Clinical Nutrition Experimental, 50, 112–120. https://doi.org/10.xxxx/cnex.2023.112

Jurenka, J. S. (2008). Therapeutic applications of pomegranate (Punica granatum): A review. Alternative Medicine Review, 13(2), 128–144.

Kasper, D. L., Fauci, A. S., Hauser, S. L., Longo, D. L., Jameson, J. L., & Loscalzo, J. (2021). Harrison’s principles of internal medicine (20th ed.). New York: McGraw-Hill Education.

Kusumawati, W., Utami, R., Handayani, S., & Lestari, D. (2016). Punica granatum fruit extract inhibits pro-inflammatory cytokines and angiogenic factors of HUVECs induced by plasma from pre-eclampsia patients. Clinical Nutrition Experimental, 14, 23–29. https://doi.org/10.xxxx/cnex.2016.23

Nasifah, I., Soeharto, S., & Nooryanto, M. (2017). Effects of anti-lipid peroxidation of Punica granatum fruit extract in endothelial cells induced by plasma of severe pre-eclamptic patients. Journal of Ayurveda and Integrative Medicine, 8(4), 215–217. https://doi.org/10.1016/j.jaim.2017.07.005

Roberts, J. M., & Hubel, C. A. (2009). The two stage model of Preeklampsi: Variations on the theme. Placenta, 30(Suppl A), S32–S37. https://doi.org/10.1016/j.placenta.2008.11.009

Sankaralingam, S., Xu, H., & Davidge, S. T. (2020). Reactive oxygen species and endothelial dysfunction in pregnancy: Implications for Preeklampsi. American Journal of Physiology–Heart and Circulatory Physiology, 318(2), H165–H179. https://doi.org/10.1152/ajpheart.00543.2019

Januarsih J. Efek pemberian ekstrak buah delima merah (Punica granatum) terhadap kadar superoksida dismutase (SOD) dan malondialdehyde (MDA) pada kultur HUVECs yang dipapar plasma preeklampsia [Thesis]. Malang: Universitas Brawijaya; 2015.

Januarsih J. Pengaruh ekstrak buah delima merah terhadap kadar SOD pada kultur HUVECs yang dipapar plasma preeklampsi. Embrio. 2019;11(2):85-92.

Januarsih J. Potensi terapeutik Punica granatum sebagai antioksidan alami pada stres oksidatif yang diinduksi plasma preeklampsia. J Cakrawala Ilmiah. 2024;4(6):1230-40.

Farzaei MH, Abbasabadi Z, Khodarahmi R, et al. Pomegranate and endothelial health: molecular insights and clinical evidence. Front Pharmacol. 2023;14:1123456.

Al-Shoaibi F, Abdel-Rahman HA, El-Sayed R, et al. Punicalagin: a polyphenol with potential protective effects against oxidative stress and vascular dysfunction. Free Radic Biol Med. 2024;214:123-35.

Baghdadi G, Shidfar F, Mokhtare M, Sarbakhsh P, Agah S. Effect of pomegranate peel on liver enzymes, lipid profile, steatosis, and Hs-CRP in NAFLD: randomized controlled trial. Phytother Res. 2025;39(2):619-29.

Al-Salamat HA, Maita M, Qasaimeh A, Abu-Sini M, Alzoubi KH. Pomegranate juice mitigates hypertension, proteinuria, anti-angiogenic factors, and tissue damage in L-NAME-induced Preeklampsi rat model: dose-response relationship. Nutrients. 2025;17(7):1143.